ABSTRACT
Uvariachamae P. Beauv. (Annonaceae) is widely distributed inAfrica, the plant species are used in traditional medicine as an anti-inflamatory, antimalarial, and analgesic. It is also used for treatment of jaundice, epilepsy and microbial infections. The powdered rootbark of U.chamaewas extracted with methanol using maceration method and the resulting crude methanol extract (CME) was solvent fractionated to give hexane (HF), chloroform (CF), ethylacetate (EF) and n-Butanol (BF) fractions. Chloroform fraction was step-wisely eluted in a silica gel packed column to afford eleven fractions S1-S12.Fraction S6was subjected to Preparative Thin Layer Chromatography (PTLC), using hexane: ethyl acetate (5:3) as solvent system led to the isolation of Quercetin. The structure of the compound was elucidated using chemical test and spectroscopic techniques (UVand 1D-NMR) and by comparison with reference spectral data.The preliminary phytochemical screening of CME revealed the presence of carbohydrates, alkaloids, flavonoids, saponins, tannins, cardiac glycosides, steroids and triterpenes. The acute toxicity study was carried out using Lorke’s method and the anticonvulsant activity was studied using maximal electroshock induced seizure test (MES) and subcutaneous pentylenetetrazole induced seizure test (Sc. PTZ). The LD50 of the crude methanol extract (CME) was estimated to be 1131.37mg/kg in mice. The crude methanol extract(CME) was found to increase the mean time of recovery from seizures in MEST compared to negative control. The absence of anticonvulsant activity in MEST suggests that CME may not be useful in the treatment of generalized tonic clonic and partial seizures. In Sc.PTZ test, the CME at dose of 30mg/kg protected 80% of mice against pentylenetetrazole and significantly (P˂ 0.05) delayed the onset of seizure. Standard antiepileptic drugs such as valproic acid are thought to produce their effects by enhancing GABA mediated inhibition in the brain. In this regard, the CME have demonstrated anticonvulsant activity and may be useful in treatment of generalized absence seizure.
ABSTRACT
This study investigated the Impact of Mastery Learning Approach (MLA) on Motivation and Academic Performance in Physics among Se...
Abstract: THE PROSPECT AND CHALLENGES OF DATA ANALYTICS IN FORENSIC ACCOUNTING INVESTIGATIONS
This research explores the prospects and ch...
The problem of power generation has rema...
ABSTRACT
This study examines the paradigm shift in business sustainability strategy from corporate soci...
Abstract
The most significant elements that enable us to understand economic growth and de...
ABSTRACT
This study was carried out to examine agriculture with special reference to Enugu North local...
THE IMPACT OF DIGITAL TRANSFORMATION ON MARKETING STRATEGIES
This study aims to: (1) investigate the impact of digital transformation on...
ABSTRACT
The primary aim of this project tilted role of electronic banking in the development of banking industries in N...
BACKGROUND OF THE STUDY
Progression is the major objective of an organisation. An organisation need to progress financia...
Abstract
Prosopis africana (Guill. & Perr.) Taub. (Mimosaceae) is the only known species of its genus found in Africa. Pharmacognosti...